Does Dietary Deoxynivalenol Modulate the Acute Phase Reaction in Endotoxaemic Pigs?—Lessons from Clinical Signs, White Blood Cell Counts, and TNF-Alpha

Toxins

Tanja Tesch²  Erik Bannert²  Jeannette Kluess²  Jana Frahm²  Susanne Kersten²  Gerhard Breves³  Lydia Renner¹  Stefan Kahlert¹  Hermann-Josef Rothkötter¹  Sven Dänicke² 

[1] Institute of Anatomy, Otto von Guericke University Magdeburg, Leipziger Str. 44, 39120 Magdeburg, Germany;Institute of Animal Nutrition, Friedrich-Loeffler Institute (FLI), Federal Research Institute of Animal Health, Bundesallee 50, 38116 Braunschweig, Germany;Institute for Physiology, University of Veterinary Medicine, Foundation, Bischofsholer Damm 15, 30173 Hannover, Germany;

关键词: pig;    deoxynivalenol;    lipopolysaccharide;    liver;    leukocytes;    clinical symptoms;    tumor necrosis factor alpha;    body core temperature;   

DOI  :  10.3390/toxins8010003

来源: mdpi

【 摘 要 】

We studied the interaction between deoxynivalenol (DON)-feeding and a subsequent pre- and post-hepatic immune stimulus with the hypothesis that the liver differently mediates the acute phase reaction (APR) in pigs. Barrows (n = 44) were divided into a DON-(4.59 mg DON/kg feed) and a control-diet group, surgically equipped with permanent catheters pre- (V. portae hepatis) and post-hepatic (V. jugularis interna) and infused either with 0.9% NaCl or LPS (7.5 µg/kg BW). Thus, combination of diet (CON vs. DON) and infusion (CON vs. LPS, jugular vs. portal) created six groups: CON_CON_jug.-CON_por., CON_CON_jug.-LPS_por., CON_LPS_jug.-CON_por., DON_CON_jug.-CON_por., DON_CON_jug.-LPS_por., DON_LPS_jug.-CON_por.. Blood samples were taken at −30, 15, 30, 45, 60, 75, 90, 120, 150, 180 min relative to infusion and analyzed for leukocytes and TNF-alpha. Concurrently, clinical signs were scored and body temperature measured during the same period. LPS as such induced a dramatic rise in TNF-alpha (p < 0.001), hyperthermia (p < 0.01), and severe leukopenia (p < 0.001). In CON-fed pigs, an earlier return to physiological base levels was observed for the clinical complex, starting at 120 min post infusionem (p < 0.05) and persisting until 180 min. DON_LPS_jug.-CON_por. resulted in a lower temperature rise (p = 0.08) compared to CON_LPS_jug.-CON_por.. In conclusion, APR resulting from a post-hepatic immune stimulus was altered by chronic DON-feeding.

【 授权许可】

CC BY   
© 2015 by the authors; licensee MDPI, Basel, Switzerland.