Maternal Supplementation of Food Ingredient (Prebiotic) or Food Contaminant (Mycotoxin) Influences Mucosal Immune System in Piglets

Nutrients

卷:12

Laurence Guzylack-Piriou¹  Stéphanie Ferret-Bernard²  Laurence Le Normand²  Gérard Savary²  Véronique Romé²  Isabelle Le Huërou-Luron²  Cindy Le Bourgot²  Fabrice Laurent³  Julie Seeboth⁴ 

[1] IALTA, IHAP, University of Toulouse, ENVT, INRAE, F-31076 Toulouse, France;

[2] INRAE, INSERM, University of Rennes, NuMeCan, Nutrition Metabolisms and Cancer, F-35000 Rennes, France;

[3] UMR ISP, INRAE, University of François Rabelais de Tours, F-37380 Nouzilly, France;

[4] UMR TOXALIM, University of Toulouse, INRA, ENVT, INP-Purpan, UPS, F-31027 Toulouse, France;

关键词: pig;    gut immunity;    intestinal antigen-presenting cell;    short-chain fructooligosaccharides;    deoxynivalenol;   

DOI  :  10.3390/nu12072115

来源: DOAJ

【 摘 要 】

The early life period is crucial for the maturation of the intestinal barrier, its immune system, and a life-long beneficial host–microbiota interaction. The study aims to assess the impact of a beneficial dietary (short-chain fructooligosaccharides, scFOS) supplementation vs. a detrimental dietary environment (such as mycotoxin deoxynivalenol, DON) on offspring intestinal immune system developmental profiles. Sows were given scFOS-supplemented or DON-contaminated diets during the last 4 weeks of gestation, whereas force-feeding piglets with DON was performed during the first week of offspring life. Intestinal antigen-presenting cell (APC) subset frequency was analyzed by flow cytometry in the Peyer’s patches and in lamina propria and the responsiveness of intestinal explants to toll-like receptor (TLR) ligands was performed using ELISA and qRT-PCR from post-natal day (PND) 10 until PND90. Perinatal exposure with scFOS did not affect the ontogenesis of APC. While it early induced inflammatory responses in piglets, scFOS further promoted the T regulatory response after TLR activation. Sow and piglet DON contamination decreased CD16+ MHCII+ APC at PND10 in lamina propria associated with IFNγ inflammation and impairment of Treg response. Our study demonstrated that maternal prebiotic supplementation and mycotoxin contamination can modulate the mucosal immune system responsiveness of offspring through different pathways.

【 授权许可】

Unknown