Genetic Diversity among Three Species of Tilapia in Egypt Detected by Random Amplified Polymorphic DNA Marker

Journal of Applied Biological Sciences

Zeinab Gaber ABD EL-HAMID¹  Karima Fathy MAHROUS¹  Heba Ahmed Mohamed ABD EL-KADER¹ 

关键词: Polymorphism;    RAPD;    PCR;    Tilapia;   

DOI  :  

学科分类：生物科学（综合）

来源: Nobel Bilim ve Arasirma Merkezi/Nobel Science and Research Cente

【 摘 要 】

GeneticdiversityofthedifferenttilapiaspeciescollectedfromCairo,GizaandFayuominEgyptwasexaminedbytheanalysisofrandomlyamplifiedpolymorphicDNA(RAPD).ThepolymorphismswithinandbetweenthreeTilapiaspecieswereassayedusing15randomprimers,and215lociwereamplifiedrangingfrom118to2556bp.Theresultsindicatedthatthepercentageofpolymorphiclocirecorded51.8%,58.6%and60.3%forOreochromisniloticus,OreochromisaureusandTilapiazillispecies,respectively.Totalgeneticdiversity(Ht)was(0.304)andtheaveragecoefficientofgeneticdifferentiation(Gst)was(0.583).ThegeneticdiversitywithinpopulationwasthehighestforT.zilli(0.249)comparedtoO.niloticusandO.aureus0.219,0.238,respectivelyandthegeneflowbetweenthreespecieswas0.357.Amongthethreepopulations,TilapiazilliappearedtobeanoutgroupwhileOreochromisniloticusandOreochromisaureusweregeneticallycloserasrevealedbyNei’sbiasedmeasureofgeneticidentityandgeneticdistancevalues.ThisstudyconcludedarelativelyhighlevelofpolymorphismandgeneticdiversitywithinandbetweenthethreetestedTilapiaspecieswhichmayberequiredfortheadaptationofthepopulationstotheenvironmentalchanges.

【 授权许可】

Unknown