Identification and differentiation of Candida species from pediatric patients by random amplified polymorphic DNA

Revista da Sociedade Brasileira de Medicina Tropical

Bruno Aragão Rocha¹  Gilda Maria Barbaro Del Negro¹  Lidia Yamamoto¹  Mariana Vitule Brito De Souza¹  Alexander Roberto Precioso¹  Thelma Suely Okay¹ 

[1] ,Universidade de São Paulo Faculdade de Medicina Department of Pediatrics

关键词: RAPD;    Candidiasis;    Candida spp;    Polymorphism;    Molecular typing;    Amplificação aleatória de DNA polimórfico;    Candidíase;    Candida spp;    Polimorfismo;    Tipagem molecular;   

DOI  :  10.1590/S0037-86822008000100001

来源: SciELO

【 摘 要 】

Thirty-four Candida isolates were analyzed by random amplified polymorphic DNA using the primer OPG-10:24 Candida albicans; 4 Candida tropicalis; 2 Candida parapsilosis; 2 Candida dubliniensis; 1 Candida glabrata and 1 Candida krusei. The UPGMA-Pearson correlation coefficient was used to calculate the genetic distance between the different Candida groupings. Samples were classified as identical (correlation of 100%); highly related samples (90%); moderately related samples (80%) and unrelated samples (< 70%). The results showed that the RAPD proposed was capable of classifying the isolates coherently (such that same species were in the same dendrogram), except for two isolates of Candida parapsilosis and the positive control (Netherlands, 1973), probably because they are now recognized as three different species. Concerning the only fluconazole-resistant Candida tropicalis isolate with a genotype that was different to the others, the data were insufficient to affirm that the only difference was the sensitivity to fluconazole. We concluded that the Random Amplified Polymorphic DNA proposed might be used to confirm Candida species identified by microbiological methods.

【 授权许可】

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