The In Vitro Alkaline Comet Assay in Genetic Toxicology

Journal of Applied Biological Sciences

Vedat ŞEKEROĞLU¹  Zeynep KOLÖREN¹  Zülal ATLI ŞEKEROĞLU¹ 

关键词: Single cell gel electrophoresis;    comet assay;    DNA damage;    genotoxicity;   

DOI  :  

学科分类：生物科学（综合）

来源: Nobel Bilim ve Arasirma Merkezi/Nobel Science and Research Cente

【 摘 要 】

Thesinglecellgel(SCG)electrophoresisorcometassayisatechniquedetectingofmultipleclassesofDNAdamagesuchassingleanddoublestrandbreaksandoxidativebasedamageandisusedforawiderangeofapplications.ThecometassayisarelativelysensitivetestwhichenablestomeasuringDNAdamagespontaneouslyandcausedbyvariousagents.Theprincipleadvantagesofthetechniqueareapplytoanycelltypefromprokaryoticandeukaryoticorganisms,culturedcellsorcellsisolatedfromtheorganismsandit.Theotheradvantagesareitsreliability,lowcosts,easeofapplication,andtheshorttimeneededtocompleteastudy.Therefore,ithasgainedinpopularityasastandardtechniqueforevaluationofDNAdamage/repair,biomonitoringandgenotoxicitystudies.Thebasicstepsofthecometassayarepreparationofmicroscopeslideslayeredwithcellsinagarose,lysis,alkalitreatment,electrophoresisunderalkalineconditions,neutralization,DNAstaining,cometvisualizationandcometscoring.Imageanalysistoolsareavailabletoanalyzevariousparametersofthecomets.Theaimofthisreviewistogiveinformationabouttheprinciplesandapplicationsofthecometassay,asensitiveandrapidtechniqueforthemeasurementofDNAdamage.

【 授权许可】

Unknown