Comparative analysis of bacterial profiles in unstimulated and stimulated saliva samples

Journal of Oral Microbiology

Daniel Belstrøm⁵  Palle Holmstrup⁵  Alexis Kokaras³  Bruce J. Paster¹  Allan Bardow²  Nils-Erik Fiehn⁴ 

[1] The Forsyth Institute, Department of Microbiology, Cambridge, MA, USADepartment of Oral Medicin, Infection & Immunity, Harvard School of Dental Medicine, Boston, MA, USA;Department of Oral Medicine, School of Dentistry, University of Copenhagen, Copenhagen, Denmark;The Forsyth Institute, Department of Microbiology, Cambridge, MA, USA;Department of Immunology & Microbiology, University of Copenhagen, Copenhagen, Denmark;Section of Periodontology and Microbiology, School of Dentistry, Faculty of Health Science, University of Copenhagen, Copenhagen, Denmark

关键词: Saliva;    HOMINGS;    bacteria;   

DOI  :  

来源: Co-Action Publishing

【 摘 要 】

Backgroundandobjective:Themicrobialprofilesofstimulatedsalivasampleshavebeenshowntodifferentiatebetweenpatientswithperiodontitis,patientswithdentalcaries,andorallyhealthyindividuals.Salivawasstimulatedtoallowforeasyandrapidcollection;however,microbialcompositionmaynotreflectthemorenatural,unstimulatedstate.Thepurposeofthisstudywastovalidatewhetherstimulatedsalivaisanadequatesurrogateforunstimulatedsalivaindeterminingsalivarymicrobiomes.Design:Unstimulated(n=20)andstimulated(n=20)salivasampleswerecollectedfrom20orallyandsystemicallyhealthy,non-smokingparticipants.SalivarybacterialprofileswereanalyzedbymeansoftheHumanOralMicrobeIdentificationusingNextGenerationSequencing(HOMINGS),andstatisticalanalysiswasperformedusingMann–WhitneytestwithBenjamini–Hochberg’scorrectionformultiplecomparison,clusteranalysis,principalcomponentanalysis,andcorrespondenceanalysis.Results:Fromatotalof40salivasamples,496probetargetswereidentifiedwithameannumberoftargetspersampleof203(range:146–303),andameannumberofprobetargetsof206and200inunstimulatedandstimulatedsalivasamples,respectively(p=0.62).Basedonallstatisticalmethodsusedforthisstudy,themicrobialprofilesofunstimulatedandstimulatedsalivasamplescollectedfromthesamepersonwerenotstatisticallysignificantlydifferent.Conclusions:Analysisofbacterialsalivaryprofilesinunstimulatedandstimulatedsalivasamplescollectedfromthesameindividualshowedcomparableresults.Thus,theresultsverifythatstimulatedsalivaisanadequatesurrogateofunstimulatedsalivaformicrobiome-relatedstudies.Keywords:saliva;HOMINGS;bacteria(Published:16March2016)Citation:JournalofOralMicrobiology2016,8:30112-http://dx.doi.org/10.3402/jom.v8.30112

【 授权许可】

Unknown