Chemical and biochemical engineering quarterly | |
Partitioning of Phenylalanine Ammonia-lyase from Rhodotorula glutinis in Aqueous Two-phase Systems of PEG/salts | |
H. Yue1  W. Wang1  Q. Yuan1  | |
关键词: Aqueous two-phase system; phenylalanine ammonia-lyase; partitioning; PEG; salt; | |
DOI : | |
来源: Croatian Society of Chemical Engineers | |
【 摘 要 】
Abstract In this work, the partitioning of phenylalanine ammonia-lyase was investigated in different systems of PEG1000, PEG2000, PEG3350, PEG6000 and PEG8000 with (NH4)2SO4, Na2SO4, Na2CO3 and potassium phosphate. The results showed that the partition of phenylalanine ammonia-lyase in PEG/salt aqueous two-phase systems was mainly influenced by hydrophobicity, excluded volume effect and salting-out effect; compared with other PEG/salt systems, PEG1000/Na2SO4 was the most effective for phenylalanine ammonia-lyase partitioning. The result further supports that PEG/salt aqueous two-phase system provides a new and applicable route for the purification of phenylalanine ammonia-lyase, as shown in our published paper.13
【 授权许可】
Unknown
【 预 览 】
Files | Size | Format | View |
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RO201912090768991ZK.pdf | 113KB | download |