Partitioning of Phenylalanine Ammonia-lyase from Rhodotorula glutinis in Aqueous Two-phase Systems of PEG/salts

Chemical and biochemical engineering quarterly

H. Yue¹  W. Wang¹  Q. Yuan¹ 

关键词: Aqueous two-phase system;    phenylalanine ammonia-lyase;    partitioning;    PEG;    salt;   

DOI  :  

来源: Croatian Society of Chemical Engineers

【 摘 要 】

Abstract In this work, the partitioning of phenylalanine ammonia-lyase was investigated in different systems of PEG1000, PEG2000, PEG3350, PEG6000 and PEG8000 with (NH4)2SO4, Na2SO4, Na2CO3 and potassium phosphate. The results showed that the partition of phenylalanine ammonia-lyase in PEG/salt aqueous two-phase systems was mainly influenced by hydrophobicity, excluded volume effect and salting-out effect; compared with other PEG/salt systems, PEG1000/Na2SO4 was the most effective for phenylalanine ammonia-lyase partitioning. The result further supports that PEG/salt aqueous two-phase system provides a new and applicable route for the purification of phenylalanine ammonia-lyase, as shown in our published paper.13

【 授权许可】

Unknown