Separation, concentration and determination of trace chloramphenicol in shrimp from different waters by using polyoxyethylene lauryl ether-salt aqueous two-phase system coupled with high-performance liquid chromatography

卷:192

Lu, Yang ; Yao, Hui ; Li, Chuang ; Han, Juan ; Tan, Zhenjiang ; Yan, Yongsheng

Jilin Normal Univ

关键词: Aqueous two-phase system;    Polyoxyethylene lauryl ether;    Trace;    Chloramphenicol;    Determine;   

DOI  :  10.1016/j.foodchem.2015.06.086

学科分类：食品科学和技术

【 摘 要 】

Polyoxyethylene lauryl ether (POELE10)-NaH2PO4 aqueous two-phase extraction system (ATPES) is coupled with HPLC to analyze chloramphenicol (CAP) in aquatic product. Response surface methodology (RSM) was adopted in the multi-factor experiment to determine the optimized conditions. The extraction efficiency of CAP (E%) is up to 99.42% under the optimal conditions, namely, the concentration of NaH2PO4, the concentration of POELE10, pH and temperature were 0.186 g.mL(-1), 0.033 g.mL(-1), 3.8 and 25 degrees C respectively. The optimal value of enrichment factor of CAP (F) was 22.56 when the concentration of NaH2PO4 was 0.192 g.mL(-1), the concentration of POELE10 was 0.024 g/ml, pH was 4.2 and temperature was 30 C. The limit of detection (LOD) and limit of quantification (LOQ) of this method are 0.8 mu g.kg(-1) and 1 mu g.kg(-1), which meet the needs of determining trace or ultratrace CAP in food. The E% and F of this technique are much better than other extraction methods. (C) 2015 Elsevier Ltd. All rights reserved.