Developmental Biology | |
Opioid Receptor-Induced GTPγ35S Binding during Mouse Development | |
Joshua F. Nitsche1  John E. Pintar1  | |
[1] Department of Neuroscience and Cell Biology, UMDNJ-Robert Wood Johnson Medical School, Piscataway, New Jersey, 08854 | |
关键词: embryonic; development; opiate; opioid receptors; GTPγ35S binding autoradiography; G-protein coupling; | |
DOI : 10.1006/dbio.2002.0855 | |
学科分类:生物科学(综合) | |
来源: Academic Press | |
【 摘 要 】
AlthoughalargesuperfamilyofG-protein-coupledreceptorsservesmultiplefunctions,littleisknownabouttheirfunctionalactivationduringontogeny.Toexaminethefunctionalactivationofthemu-opioidreceptor(MOR)andthedelta-opioidreceptor(DOR)duringdevelopment,sectionsofmouseembryosandfetusesfrome11.5untilbirthweretreatedwithDAMGOandDPDPE,respectively,andtheabilityofthesedrugstoinduceG-proteincouplingwasassessedbyusingGTPγ35Sbindingautoradiography.MORactivationwasfirstdetectedinthecaudate-putamen(CPU)ate12.5,andbye15.5,activityhadnotonlyincreasedinthisregionbutalsoexpandedtoincludethemidbrain,medialhabenula,hypothalamus,pons,andmedulla.DORactivityfirstappearedate17.5inthehypothalamus,pons,medialhabenula,andmedullaandatp1intheCPUatlevelsnoticeablylessthanthoseoftheMOR.Ingeneral,MORandDORactivationlaggedonlyslightlybehindtheappearanceofMOR-1andDOR-1mRNAbutdelayedactivationwasparticularlypronouncedinthetrigeminalganglia,whereMOR-1geneexpressionwasfirstdetectedate13.5,butMORactivitywasnotobservedevenatbirth.Thus,thedatademonstratetemporalandoftenregion-specificdifferencesintheappearanceandmagnitudeoffunctionalactivityincellgroupsexpressingeithertheMOR-1orDOR-1genes,suggestingthatinteractionbetweentheopioidreceptors,G-proteins,andothersignalingcofactorsisdevelopmentallyregulated.
【 授权许可】
Unknown
【 预 览 】
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