Opioid Receptor-Induced GTPγ35S Binding during Mouse Development

Developmental Biology

Joshua F. Nitsche¹  John E. Pintar¹ 

[1] Department of Neuroscience and Cell Biology, UMDNJ-Robert Wood Johnson Medical School, Piscataway, New Jersey, 08854

关键词: embryonic;    development;    opiate;    opioid receptors;    GTPγ35S binding autoradiography;    G-protein coupling;   

DOI  :  10.1006/dbio.2002.0855

学科分类：生物科学（综合）

来源: Academic Press

【 摘 要 】

AlthoughalargesuperfamilyofG-protein-coupledreceptorsservesmultiplefunctions,littleisknownabouttheirfunctionalactivationduringontogeny.Toexaminethefunctionalactivationofthemu-opioidreceptor(MOR)andthedelta-opioidreceptor(DOR)duringdevelopment,sectionsofmouseembryosandfetusesfrome11.5untilbirthweretreatedwithDAMGOandDPDPE,respectively,andtheabilityofthesedrugstoinduceG-proteincouplingwasassessedbyusingGTPγ35Sbindingautoradiography.MORactivationwasfirstdetectedinthecaudate-putamen(CPU)ate12.5,andbye15.5,activityhadnotonlyincreasedinthisregionbutalsoexpandedtoincludethemidbrain,medialhabenula,hypothalamus,pons,andmedulla.DORactivityfirstappearedate17.5inthehypothalamus,pons,medialhabenula,andmedullaandatp1intheCPUatlevelsnoticeablylessthanthoseoftheMOR.Ingeneral,MORandDORactivationlaggedonlyslightlybehindtheappearanceofMOR-1andDOR-1mRNAbutdelayedactivationwasparticularlypronouncedinthetrigeminalganglia,whereMOR-1geneexpressionwasfirstdetectedate13.5,butMORactivitywasnotobservedevenatbirth.Thus,thedatademonstratetemporalandoftenregion-specificdifferencesintheappearanceandmagnitudeoffunctionalactivityincellgroupsexpressingeithertheMOR-1orDOR-1genes,suggestingthatinteractionbetweentheopioidreceptors,G-proteins,andothersignalingcofactorsisdevelopmentallyregulated.

【 授权许可】

Unknown