Neurogenin1 Defines Zebrafish Cranial Sensory Ganglia Precursors

Developmental Biology

David W. Raible¹  Peter Andermann¹  Josette Ungos¹ 

[1] Department of Biological Structure, University of Washington, Seattle, Washington, 98195-7420

关键词: trigeminal placode;    lateral line;    epibranchial placode;    otic placode;    neurogenin;    bHLH transcription factor;    morpholino oligonucleotides;   

DOI  :  10.1006/dbio.2002.0820

学科分类：生物科学（综合）

来源: Academic Press

【 摘 要 】

Cellsdelaminatefromepithelialplacodestoformsensorygangliainthevertebratehead.Wedescribetheformationofcranialneurogenicplacodesinthezebrafish,Daniorerio,usingbHLHtranscriptionfactorsasmolecularmarkers.Asingleneurogeningene,neurogenin1(ngn1),isrequiredforthedevelopmentofallzebrafishcranialganglia,whichcontrastswithotherdescribedvertebrates.Expressionofngn1delineateszebrafishganglionicplacodes,includingtrigeminal,lateralline,andepibranchialplacodes.Inaddition,ngn1isexpressedinasubsetofcellswithintheoticvesiclethatwilldelaminatetoformtheoctaval(statoacoustic)ganglion.Thetrigeminalplacodeisthefirsttodifferentiate,andformsjustlateralandadjacenttotheneuralcrest.Expressionofngn1istransientandprefiguresexpressionofarelatedbHLHtranscriptionfactor,neuroD.Interferingwithngn1functionusingaspecificantisensemorpholinooligonucleotideblocksdifferentiationofallcranialgangliabutnotassociatedglialcells.Laterallinesensoryneuromastsdevelopindependentlyofngn1function,suggestingthattwoderivativesoflaterallineplacodes,gangliaandmigratingprimordia,areunderseparategeneticcontrol.

【 授权许可】

Unknown