Retrograde Ret signaling controls sensory pioneer axon outgrowth

eLife

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[1] Department of Cell, Developmental, and Cancer Biology, Oregon Health & Science University, Portland, United States;Department of Cell, Developmental, and Cancer Biology, Oregon Health & Science University, Portland, United States;Neuroscience Graduate Program, Oregon Health & Science University, Portland, United States;

关键词: pioneer neuron;    growth cone;    lateral line;    axon growth;    Ret;    axonal transport;    Zebrafish;   

DOI  :  10.7554/eLife.46092

来源: publisher

【 摘 要 】

10.7554/eLife.46092.001The trafficking mechanisms and transcriptional targets downstream of long-range neurotrophic factor ligand/receptor signaling that promote axon growth are incompletely understood. Zebrafish carrying a null mutation in a neurotrophic factor receptor, Ret, displayed defects in peripheral sensory axon growth cone morphology and dynamics. Ret receptor was highly enriched in sensory pioneer neurons and Ret51 isoform was required for pioneer axon outgrowth. Loss-of-function of a cargo adaptor, Jip3, partially phenocopied Ret axonal defects, led to accumulation of activated Ret in pioneer growth cones, and reduced retrograde Ret51 transport. Jip3 and Ret51 were also retrogradely co-transported, ultimately suggesting Jip3 is a retrograde adapter of active Ret51. Finally, loss of Ret reduced transcription and growth cone localization of Myosin-X, an initiator of filopodial formation. These results show a specific role for Ret51 in pioneer axon growth, and suggest a critical role for long-range retrograde Ret signaling in regulating growth cone dynamics through downstream transcriptional changes.

【 授权许可】

CC BY