The Role of the Ets Domain Transcription Factor Erm in Modulating Differentiation of Neural Crest Stem Cells

Developmental Biology

Christian Paratore¹  Guya Brugnoli¹  Hye-Youn Lee¹  Lukas Sommer¹  Ueli Suter¹ 

[1] Institute of Cell Biology, Swiss Federal Institute of Technology, ETH-Hönggerberg, CH-8093, Zürich, Switzerland

关键词: PNS;    neural crest stem cells;    glia;    proliferation;    neuronal fate;    Erm;    Ets domain transcription factor;   

DOI  :  10.1006/dbio.2002.0795

学科分类：生物科学（综合）

来源: Academic Press

【 摘 要 】

ThetranscriptionfactorErmisamemberofthePea3subfamilyofEtsdomainproteinsthatisexpressedinmultipotentneuralcrestcells,peripheralneurons,andsatelliteglia.AspecificroleofErmduringdevelopmenthasnotyetbeenestablished.WeaddressedthefunctionofErminneuralcrestdevelopmentbyforcedexpressionofadominant-negativeformofErm.FunctionalinhibitionofErminneuralcrestcellsinterferedwithneuronalfatedecision,whileprogenitorsurvivalandproliferationwerenotaffected.Incontrast,blockingErmfunctioninneuralcreststemcellsdidnotinfluencetheirabilitytoadoptaglialfate,independentoftheglia-inducingsignal.Furthermore,glialsurvivalanddifferentiationwerenormal.However,theproliferationratewasdrasticallydiminishedinglialcells,suggestingaglia-specificroleofErmincontrollingcellcycleprogression.Thus,incontrasttoothermembersofthePea3subfamilythatareinvolvedinlatestepsofneurogenesis,Ermappearstoberequiredinearlyneuralcrestdevelopment.Moreover,ourdatapointtomultiple,lineage-specificrolesofErminneuralcreststemcellsandtheirderivatives,suggestingthatErmfunctionisdependentonthecellintrinsicandextrinsiccontext.

【 授权许可】

Unknown