Twist Function Is Required for the Morphogenesis of the Cephalic Neural Tube and the Differentiation of the Cranial Neural Crest Cells in the Mouse Embryo

Developmental Biology

Richard R. Behringer¹  Kenneth Soo¹  Meredith P. O'Rourke¹  Nicole Wong¹  Patrick P.L. Tam¹  Poh-Lynn Khoo¹  Kirsten A. Steiner¹ 

[1] Embryology Unit, Children's Medical Research Institute, Locked Bag 23, Wentworthville, NSW 2145, Australia

关键词: Twist;    dorsoventral patterning;    neural tube;    neural crest cells;    tissue potency;    cell migration;   

DOI  :  10.1006/dbio.2002.0699

学科分类：生物科学（综合）

来源: Academic Press

【 摘 要 】

LossofTwistfunctioninthecranialmesenchymeofthemouseembryocausesfailureofclosureofthecephalicneuraltubeandmalformationofthebranchialarches.IntheTwist−/−embryo,theexpressionofmolecularmarkersthatsignifydorsalforebraintissuesiseitherabsentorreduced,butthoseassociatedwithventraltissuesdisplayexpandeddomainsofexpression.Dorsoventralorganizationofthemid-andhindbrainandtheanterior–posteriorpatternoftheneuraltubearenotaffected.IntheTwist−/−embryo,neuralcrestcellsstrayfromthesubectodermalmigratorypathandthelate-migratingsubpopulationinvadesthecell-freezoneseparatingstreamsofcellsgoingtothefirstandsecondbranchialarches.CelltransplantationstudiesrevealthatTwistactivityisrequiredinthecranialmesenchymefordirectingthemigrationoftheneuralcrestcells,aswellasintheneuralcrestcellswithinthefirstbranchialarchtoachievecorrectlocalization.Twistisalsorequiredfortheproperdifferentiationofthefirstarchtissuesintobone,muscle,andteeth.

【 授权许可】

Unknown