Bmp Activity Gradient Regulates Convergent Extension during Zebrafish Gastrulation

Developmental Biology

Dina C. Myers¹  Lilianna Solnica-Krezel¹  Diane S. Sepich¹ 

[1] Department of Biological Sciences, Vanderbilt University, VU Station B 351634, Nashville, Tennessee, 37235-1634

关键词: convergence;    extension;    morphology;    dorsoventral patterning;    morphogenesis;   

DOI  :  10.1006/dbio.2001.0523

学科分类：生物科学（综合）

来源: Academic Press

【 摘 要 】

Duringvertebrategastrulation,aventraltodorsalgradientofbonemorphogeneticprotein(Bmp)activityestablishescellfates.Concomitantly,convergentextensionmovementsnarrowgermlayersmediolaterallywhilelengtheningthemanteroposteriorly.Here,bymeasuringmovementsofcellpopulationsinvivo,werevealthepresenceofthreedomainsofconvergentextensionmovementsinzebrafishgastrula.Ventrally,convergenceandextensionmovementsareabsent.Lateralcellpopulationsconvergeandextendatincreasingspeeduntiltheyreachthedorsaldomainwhereconvergencespeedslowsbutextensionremainsstrong.Usingdorsalizedandventralizedmutants,wedemonstratethatthesedomainsarespecifiedbytheBmpactivitygradient.InvivocellmorphologyandbehavioranalysesindicatedthatlowlevelsofBmpactivitymightpromoteextensionwithlittleconvergencebyallowingmediolateralcellelongationanddorsallybiasedintercalation.Further,singlecellmovementanalysesrevealedthatthehighventrallevelsofBmpactivitypromoteepibolicmigrationofcellsintothetailbud,increasingtailformationattheexpenseofheadandtrunk.WeshowthathighBmpactivitylimitsconvergenceandextensionbynegativelyregulatingexpressionofthewnt11(silberblick)andwnt5a(pipetail)genes,whicharerequiredforconvergentextensionbutnotcellfatespecification.Therefore,duringvertebrategastrulation,asinglegradientofBmpactivity,whichspecifiescellfates,alsoregulatesthemorphogeneticprocessofconvergentextension.

【 授权许可】

Unknown