Developmental Biology | |
Differential Effects of Specific Phosphodiesterase Isoenzyme Inhibitors on Bovine Oocyte Meiotic Maturation | |
David T. Armstrong1  Robert B. Gilchrist1  Rebecca E. Thomas1  | |
[1] Reproductive Medicine Unit, Department of Obstetrics and Gynaecology, The University of Adelaide, The Queen Elizabeth Hospital, 5011, Adelaide, Australia | |
关键词: oocyte; meiotic maturation; meiotic inhibition; cAMP; phosphodiesterase; granulosa cell; | |
DOI : 10.1006/dbio.2002.0609 | |
学科分类:生物科学(综合) | |
来源: Academic Press | |
【 摘 要 】
ThedifferentialregulationofcAMPlevelswithintheoocyteandsomatic(cumulus)cellcompartmentsofthebovinefollicle,andthesubsequentregulationofoocytemeioticmaturationwasexaminedthroughspecificcell-typelocalisationofphosphodiesterases(PDEs).SelectivePDEinhibitorswereusedtomodulatecAMPlevelsineachofthetwofollicularcompartmentsandtoexaminetheireffectsonoocytemeioticmaturation.Ovarieswereobtainedfromanabattoirandcumulus-oocytecomplexes(COC)wereaspiratedfromantralfolliclesintoculturemediumsupplementedwith4mg/mlBSAand2mM3-isobutyl-1-methylxanthine(IBMX).COC,denudedoocytes(DO),ormuralgranulosacells(MGC)wereculturedeitherwithorwithoutforskolinorFSH,inthepresenceofspecificPDEinhibitors;eithermilrinone(PDE3inhibitor),cilostamide(PDE3inhibitor),orrolipram(PDE4inhibitor).COC/DOcultureswereassessedformeioticprogressionandcAMPcontent,andMGCforcAMPproduction.Thetype3PDEinhibitor,butnotthetype4,preventedspontaneousmeioticmaturationandelevatedintraoocytecAMPincultureddenudedoocytes.Incontrast,thetype4PDEinhibitorhadnoeffectontheoocyte,butelevatedmuralgranulosaandcumuluscellcAMPproduction.TheresultsofthisstudyindicatethatspecificPDEsubtypesaredifferentiallylocalisedwithinthetwocompartmentsofthebovinefollicle—thetype3PDEintheoocyteandthetype4PDEinthegranulosacells.Inaddition,oocytecAMPlevelsareprimarilyregulatedinbovineoocytesbyitsdegradationbyPDE,whereasgranulosacellcAMPlevelsarecontrolledmainlybyactiveadenylatecyclase,withbothsourcesabletoparticipateinoocytemeioticregulation.
【 授权许可】
Unknown
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