Differential Effects of Specific Phosphodiesterase Isoenzyme Inhibitors on Bovine Oocyte Meiotic Maturation

Developmental Biology

David T. Armstrong¹  Robert B. Gilchrist¹  Rebecca E. Thomas¹ 

[1] Reproductive Medicine Unit, Department of Obstetrics and Gynaecology, The University of Adelaide, The Queen Elizabeth Hospital, 5011, Adelaide, Australia

关键词: oocyte;    meiotic maturation;    meiotic inhibition;    cAMP;    phosphodiesterase;    granulosa cell;   

DOI  :  10.1006/dbio.2002.0609

学科分类：生物科学（综合）

来源: Academic Press

【 摘 要 】

ThedifferentialregulationofcAMPlevelswithintheoocyteandsomatic(cumulus)cellcompartmentsofthebovinefollicle,andthesubsequentregulationofoocytemeioticmaturationwasexaminedthroughspecificcell-typelocalisationofphosphodiesterases(PDEs).SelectivePDEinhibitorswereusedtomodulatecAMPlevelsineachofthetwofollicularcompartmentsandtoexaminetheireffectsonoocytemeioticmaturation.Ovarieswereobtainedfromanabattoirandcumulus-oocytecomplexes(COC)wereaspiratedfromantralfolliclesintoculturemediumsupplementedwith4mg/mlBSAand2mM3-isobutyl-1-methylxanthine(IBMX).COC,denudedoocytes(DO),ormuralgranulosacells(MGC)wereculturedeitherwithorwithoutforskolinorFSH,inthepresenceofspecificPDEinhibitors;eithermilrinone(PDE3inhibitor),cilostamide(PDE3inhibitor),orrolipram(PDE4inhibitor).COC/DOcultureswereassessedformeioticprogressionandcAMPcontent,andMGCforcAMPproduction.Thetype3PDEinhibitor,butnotthetype4,preventedspontaneousmeioticmaturationandelevatedintraoocytecAMPincultureddenudedoocytes.Incontrast,thetype4PDEinhibitorhadnoeffectontheoocyte,butelevatedmuralgranulosaandcumuluscellcAMPproduction.TheresultsofthisstudyindicatethatspecificPDEsubtypesaredifferentiallylocalisedwithinthetwocompartmentsofthebovinefollicle—thetype3PDEintheoocyteandthetype4PDEinthegranulosacells.Inaddition,oocytecAMPlevelsareprimarilyregulatedinbovineoocytesbyitsdegradationbyPDE,whereasgranulosacellcAMPlevelsarecontrolledmainlybyactiveadenylatecyclase,withbothsourcesabletoparticipateinoocytemeioticregulation.

【 授权许可】

Unknown