Follicle-Stimulating Hormone Induces a Gap Junction-Dependent Dynamic Change in [cAMP] and Protein Kinase A in Mammalian Oocytes

Developmental Biology

Karl Swann¹  Rachel J. Webb¹  John Carroll¹  Felicity Marshall¹ 

[1] Department of Physiology, University College London, Gower Street, London, WC1E 6BT, United Kingdom

关键词: cAMP;    FlCRhR;    PKA;    ATP;    AKAP;    oocyte;    gap junction;    ovary;    mouse;   

DOI  :  10.1006/dbio.2002.0630

学科分类：生物科学（综合）

来源: Academic Press

【 摘 要 】

Thesecondmessengercyclicadenosine5′monophosphate(cAMP)hasbeenimplicatedincontrollingmeioticmaturation.Todate,therehavebeennodirectmeasurementsofcAMPinlivingmammalianoocytes.Here,wehaveusedthefluorescentlylabelledcAMP-dependentproteinkinaseA(PKA),FlCRhR,tomonitorcAMPinmouseoocytes.Incumulus-enclosedoocytes,follicle-stimulatinghormone(FSH)stimulatedanincreaseintheoocyte[cAMP]thatwaspreventedbyusingthegapjunctioninhibitor,carbenoxolone.TheFSH-inducedincreaseinoocyte[cAMP]wassuppressedinatime-dependentmannerbypriorexposuretoATP,whileepidermalgrowthfactorhadnoeffectonbasalorstimulatedlevelsofcAMP.Finally,usingconfocalmicroscopy,weshowthattheregulatoryandcatalyticsubunitsofthemicroinjectedPKAaredistributedinapunctatemannerwithastrongeraccumulationintheperinuclearregion.Onanincreasein[cAMP],inresponsetophosphodiesteraseinhibitionorFSH,thecatalyticsubunitdiffusedthroughoutthecytoplasmandgerminalvesicle,whiletheregulatorysubunitremainedanchored.TheseexperimentsshowthatincreasesincAMPinovariansomaticcellsarecommunicatedviagapjunctionstotheoocyte,whereitcanleadtoaredistributionofthecatalyticsubunitofPKA.

【 授权许可】

Unknown