Follistatin Regulates Bone Morphogenetic Protein-7 (BMP-7) Activity to Stimulate Embryonic Muscle Growth

Developmental Biology

Helge Amthor¹  Bodo Christ¹  Emily Lang¹  Fiza Rashid-Doubell¹  C.Fred Kemp¹  Ketan Patel¹ 

[1] Institute of Anatomy, University of Freiburg, P.O. Box 111, D-79001, Freiburg, Germany

关键词: Follistatin;    BMP;    Noggin;    embryo;    muscle development;    Pax-3;    MyoD;    Biacore;   

DOI  :  10.1006/dbio.2001.0555

学科分类：生物科学（综合）

来源: Academic Press

【 摘 要 】

Bonemorphogeneticproteins(BMPs)caneitherpromotegrowthofembryonicmusclebyexpandingthePax-3-expressingmuscleprecursorpopulationorrestrictitsdevelopmentbyinducingapoptosis.Follistatin,aproposedBMPantagonist,isexpressedinembryonicmuscle.DeficiencyinFollistatinresultsinmuscledefectsandpostnatalasphyxia.Here,wereportthatduringchicklimbdevelopmentFollistatinenhancesBMP-7actiontoinducemusclegrowthbutpreventstheabilityofBMP-7toinduceapoptosisandmuscleloss.Follistatin,unlikeanotherBMP-bindingprotein,Noggin,promotesPax-3expressionandtransientlydelaysmuscledifferentiationandthusexertsproliferativesignallingduringmuscledevelopment.WeprovidedatawhichshowthatFollistatinbindsBMP-7andBMP-2atlowaffinitiesandthatthebindingisreversible.ThesedatasuggestthatFollistatinactstopresentBMPstomyogeniccellsataconcentrationthatpermitsstimulationofembryonicmusclegrowth.

【 授权许可】

Unknown