Regulation of Stage-Specific Nuclear Translocation of Dnmt1o during Preimplantation Mouse Development

Developmental Biology

Richard M. Schultz¹  Marisa S. Bartolomei¹  Adam S. Doherty¹ 

[1] Department of Biology, Department of Cell and Developmental Biology, University of Pennsylvania, Philadelphia, Pennsylvania, 19104

关键词: DNA methylation;    DNA methyltransferase;    compaction;    preimplantation embryo;   

DOI  :  10.1006/dbio.2001.0534

学科分类：生物科学（综合）

来源: Academic Press

【 摘 要 】

DNAmethylationofCpGdinucleotidesbyDNAmethyltransferase1isimplicatedintheregulationoftranscriptionand,inparticular,thetranscriptionofimprintedgenes.Althoughtheoocyte-specificformofDnmt1(Dnmt1o)possessesafunctionalnuclearlocalizationsignal,itispredominantlylocalizedinthecytoplasmoftheoocyteandpreimplantationmouseembryobutundergoesatransientnuclearlocalizationduringtheeight-cellstage,whentheembryosundergocompaction.WereportherethatDnmt1oislikelyretainedinthecytoplasmbyanactiveprocess,since∼70%ofDNAmethyltransferaseactivityisretainedfollowingpermeabilizationproceduresthatresultinthereleaseof∼75%ofoocyte/embryoprotein.Treatmentoftheembryoswithagentsthatdisrupteithermicrofilamentsormicrotubuleshaslittle,ifany,effectontheretentionofDnmt1oinpermeabilizedembryos.WhileDnmt1odoesnotcolocalizewitheithermitochondriaorendoplasmicreticulum,itdoescolocalizewithannexinV,whichisknowntointeractwithDnmt1o.WealsoreportthatthetimingofnuclearentryofDnmt1oduringtheeight-cellstageisindependentofDNAreplication,transcription,andproteinsynthesis,aswellascompaction,cellcontact,andcytokinesis.Thetimeofnuclearentry,therefore,appearslinkedtothetimefollowingfertilization,whichsuggeststhatamolecularclockgovernsthetimeofnuclearimport.

【 授权许可】

Unknown