Chicken spermatogenesis is accompanied by a genomic‐wide loss of DNA methylation

FEBS Letters

Mezquita, Cristóbal¹  Rocamora, Nativitat¹ 

[1] Department of Physiological Sciences, Molecular Genetics Research Group, Faculty of Medicine, University of Barcelona, Casanova 143, 08036 Barcelona, Spain

关键词: DNA methylation;    Hypomethylation;    Spermatogenesis;    DNA methyltransferase;    Enzyme activity;    HPLC;    high-performance liquid chromatography;    DTT;    dithiothreitol;    PMSF;    phenylmethylsulfonyl fluoride;    [3H]AdoMet;    S-adenosyl-L-[methyl-3H]-methionine;   

DOI  :  10.1016/0014-5793(89)81382-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Renaturation kinetics of DNA obtained from chicken testis cell nuclei separated by sedimentation at unit gravity showed that the undermethylation, previously observed in meiotic and postmeiotic cells, is not a peculiarity of repetitive sequences, but is also a feature of unique sequences. The large proportion of slowly renaturing, intermediately renaturing and rapidly renaturing DNAs contain 27, 32 and 31% less methylcytosines in meiotic and postmeiotic cells than the corresponding fractions of premeiotic cells. DNA methyltransferase activity is lower in meiotic and postmeiotic cells containing undermethylated DNA than in immature testis, enriched in spermatogonia, with higher levels of DNA methylation.

【 授权许可】

Unknown