Study of the Murine Allantois by Allantoic Explants

Developmental Biology

Roselynn Temkin¹  Shannon Gifford¹  Jacalyn McHugh¹  Karen M Downs¹ 

[1] Department of Anatomy, University of Wisconsin–Madison Medical School, 1300 University Avenue, Madison, Wisconsin, 53706

关键词: allantois;    angioblasts;    chorion;    embryos;    endothelium;    flk1;    flt1;    immunohistochemistry;    in vitro;    lacZ;    mesenchyme;    mesothelium;    mouse;    placenta;    smooth muscle;    tie1;    tie2;    transplantation;    vasculogenesis;    VCAM1;    VEGF;   

DOI  :  10.1006/dbio.2001.0227

学科分类：生物科学（综合）

来源: Academic Press

【 摘 要 】

Themurineallantoiswillbecometheumbilicalarteryandveinofthechorioallantoicplacenta.Inpreviousstudies,growthanddifferentiationoftheallantoishadbeenelucidatedinwholeembryos.Inthisstudy,theextenttowhichexplantedallantoisesgrowanddifferentiateoutsideoftheconceptuswasinvestigated.Theexplantmodelwasthenusedtoelucidatecellandgrowthfactorrequirementsinallantoicdevelopment.Earlyheadfold-stagemurineallantoiseswereexplanteddirectlyontotissuecultureplasticorsuspendedintesttubes.Explantedallantoisesvascularizedwithdistal-to-proximalpolarity,theyexhibitedmanyofthesamesignalingfactorsusedbythevitellineandcardiovascularsystems,andtheycontainedatleastthreecelltypeswhoseidentity,geneexpressionprofiles,topographicalassociations,andbehaviorresembledthoseofintactallantoises.DiIlabelingfurtherrevealedthatisolatedallantoisesgrewandvascularizedintheabsenceofsignificantcellmingling,therebysupportingamodelofmesodermaldifferentiationintheallantoisthatisposition-andpossiblyage-dependent.Manipulationofallantoicexplantsbyvaryinggrowthmediademonstratedthattheallantoicendothelialcelllineage,likethatofotherembryonicvasculatures,isresponsivetoVEGF164.AlthoughVEGF164wasrequiredforbothsurvivalandproliferationofallantoicangioblasts,itwasnotsufficienttoinduceappropriateepithelializationofthesecells.Rather,otherVEGFisoformsand/ortheoutersheathofmesothelium,whosemaintenancedidnotappeartobedependentuponendothelium,mayalsoplayimportantroles.Onthebasisofthesefindings,weproposemurineallantoicexplantsasanewtoolforsheddinglightnotonlyonallantoicdevelopment,butforelucidatinguniversalmechanismsofbloodvesselformation,includingvascularsupportingcells,eitherintheintactorganismorinexistinginvitrosystems.

【 授权许可】

Unknown