VEGF Regulates Cell Behavior during Vasculogenesis☆

Developmental Biology

Amanda LaRue¹  Christopher J. Drake¹  Napoleone Ferrara¹  Charles D. Little¹ 

[1] Department of Cell Biology and the Cardiovascular Developmental Biology Center, Medical University of South Carolina, Charleston, South Carolina, 29425

关键词: VEGF;    vasculogenesis;    angioblasts;    morphogenesis;   

DOI  :  10.1006/dbio.2000.9744

学科分类：生物科学（综合）

来源: Academic Press

【 摘 要 】

Prominentamongmoleculesthatcontrolneovascularprocessesisvascularendothelialgrowthfactor(VEGF).TheVEGFligandscompriseafamilyofwell-studiedmitogens/permeabilityfactorsthatbindcellsurfacereceptortyrosinekinases.TargetsincludeVEGFreceptor-1/Flt1andVEGFreceptor-2/Flk1.MicelackinggenesforVEGFligandorVEGFreceptor-2dieearlyingestation,makingitdifficulttodeterminetheprecisenatureofunderlyingendothelialcellularbehavior(s).Toexaminetheeffect(s)ofVEGFsignalingoncellbehaviorindetail,weconductedloss-of-functionstudiesusingavianembryos.InjectionofsolubleVEGFR-1resultsinmalformedvascularnetworksandtheabsenceoflargevessels.Inthemostseverecasesembryosexhibitedvascularatresia.Closelyassociatedwiththealteredphenotypewasaclearendothelialcellresponse—amarkeddecreaseincellprotrusiveactivity.Further,wedemonstratethatVEGFgainoffunctionstrikinglyincreasedcellprotrusiveactivity.Together,ourdatashowthatVEGF/VEGFreceptorsignalingregulatesendothelialcellprotrusiveactivity,akeydeterminantofbloodvesselmorphogenesis.WeproposethatVEGFfunctionsasaninstructivemoleculeduringdenovobloodvesselmorphogenesis.

【 授权许可】

Unknown