Drosophila Single-minded Represses Gene Transcription by Activating the Expression of Repressive Factors

Developmental Biology

Patricia Estes¹  Jack Mosher¹  Stephen T. Crews¹ 

[1] Department of Biochemistry and Biophysics, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, 27599-7260

关键词: bHLH-PAS;    CNS midline;    neuroectoderm;    single-minded;    ventral nervous system defective;    tango.;   

DOI  :  10.1006/dbio.2001.0174

学科分类：生物科学（综合）

来源: Academic Press

【 摘 要 】

TheDrosophilasingle-mindedgenecontrolsCNSmidlinecelldevelopmentbybothactivatingmidlinegeneexpressionandrepressinglateralCNSgeneexpressioninthemidlinecells.ThemechanismbywhichSingle-mindedrepressestranscriptionwasexaminedusingtheventralnervoussystemdefectivegeneasatargetgene.Transgenic-lacZanalysisofconstructscontainingfragmentsoftheventralnervoussystemdefectiveregulatoryregionidentifiedsequencesrequiredforlateralCNStranscriptionandmidlinerepression.EliminationofSingle-minded:Tangobindingsiteswithintheventralnervoussystemdefectivegenedidnotaffectmidlinerepression.MutantsofSingle-mindedthatremovedtheDNAbindingandtranscriptionalactivationregionsabolishedventralnervoussystemdefectiverepression,aswellastranscriptionalactivationofothergenes.ThereplacementoftheSingle-mindedtranscriptionalactivationregionwithaheterologousVP16transcriptionalactivationregionrestoredtheabilityofSingle-mindedtobothactivateandrepresstranscription.TheseresultsindicatethatSingle-mindedindirectlyrepressestranscriptionbyactivatingtheexpressionofrepressivefactors.Single-mindedprovidesamodelsystemforhowregulatoryproteinsthatactonlyastranscriptionalactivatorscancontrollineage-specifictranscriptioninbothpositiveandnegativemodes.

【 授权许可】

Unknown