Novel Behavioral and Developmental Defects Associated with Drosophila single-minded

Developmental Biology

Roland Strauss¹  Dan C Lau¹  Stephen T Crews¹  Georg Steffes¹  Jan Pielage¹  Beth A Parente¹  Christian Klämbt¹ 

[1] Institut für Neurobiologie, Universität Münster, Badestrasse 9, D-48149, Münster, Germany

关键词: single-minded;    Drosophila;    CNS;    midline;    genital disc;    brain;    behavior;   

DOI  :  10.1006/dbio.2002.0770

学科分类：生物科学（综合）

来源: Academic Press

【 摘 要 】

InDrosophila,thedevelopmentofthemidlinecellsoftheembryonicventralnervecorddependsonthefunctionofthebHLH-PAStranscriptionfactorSingle-minded(Sim).Theexpressiondomainofsim,however,isalsofoundanteriorandposteriortothedevelopingventralcordthroughoutthegermband.Indeed,mutationsinsimwereidentifiedbasedontheircharacteristiccuticlephenotype.Eightabdominalsegments(A1–A8)canbeeasilyseeninthelarvalcuticle,whilethreemorecanbeidentifiedduringembryogenesis.CellslocatedinA8–A10giverisetotheformationofthegenitalimaginaldiscs,andahighlymodifiedA11segmentgivesrisetotheanalpadsthatflanktheanus.simisexpressedinallthesesegmentsandisrequiredfortheformationofboththeanalpadsandthegenitalimaginaldiscs.Anewtemperature-sensitivesimalleleallowedanassessmentofpossiblepostembryonicfunction(s)ofsim.Reductionofsimfunctionbelowa50%thresholdleadstosterileflieswithmarkedbehavioraldeficits.Mostmutantsimflieswereonlyabletowalkincircles.Furtheranalysesindicatedthatthisphenotypeislikelyduetodefectsinthebraincentralcomplex.Thisbrainregion,whichhaspreviouslybeenimplicatedinthecontrolofwalkingbehavior,expresseshighlevelsofnuclearSimproteininthreeclustersofneuronsineachcentralbrainhemisphere.AdditionalSimlocalizationinthemedullaryandlaminarneuronsoftheopticlobesmaycorrelatewiththepresenceofectopicaxonbundlesobservedintheopticlobesofsimmutantflies.

【 授权许可】

Unknown