Tracing the Progeny of the Aortic Hemangioblast in the Avian Embryo

Developmental Biology

V. Brajeul¹  F. Dieterlen-Lièvre¹  R. Gautier¹  Thierry Jaffredo¹ 

[1] Institut d'Embryologie Cellulaire et Moléculaire, CNRS, Collège de France, 49 bis, Avenue de la Belle Gabrielle, 94736, Nogent-sur-Marne Cedex, France

关键词: chick embryo;    hematopoiesis;    hemangioblast;    CD45;    retroviral vectors;   

DOI  :  10.1006/dbio.2000.9799

学科分类：生物科学（综合）

来源: Academic Press

【 摘 要 】

Apopulationofhematopoieticprogenitorsbecomescommittedwithintheembryoproperintheflooroftheaorta(P-Sp/AGMinthemouse).Inbirds,thisfirstaspectofintraembryonichematopoiesisisprominentduringembryonicday3(E3)asendothelium-associated“intra-aorticclusters.”BetweenE6andE8,diffusehematopoiesisthenoccursas“para-aorticfoci”locatedinthedorsalmesenteryventraltotheaorta.Thesefociarenotassociatedwithendothelium.Whetherthesetwohematopoieticcellpopulationsarisefromdistinctorcommonprogenitorsisnotknown.Wecouldrecentlytracebacktheoriginofintra-aorticclustersintheavianembryobylabelingaorticendothelialcells(EC)invivowithacetylatedlow-densitylipoproteins.Thisapproachestablishedthederivationofearlyintraembryonichemopoieticcellsfromtheendothelium,butdidnotindicatehowlongduringontogenysucharelationshipmayexist,sincetheprogenyofEClabeledatE2couldbetracedfor1–2daysatmost.Herewereportthat,whenE2aorticECswereinfectedpriortotheformationofintra-aorticclusterswithanonreplicativeLacZ-bearingretroviralvector,numerouscellswerelabeledinthepara-aorticfociatE6.Incontrast,whentheretroviralvectorwasinoculatedatE4ratherthanE2,thatis,afterthedisappearanceofintra-aorticclusters,nocellsinthepara-aorticfociwerelabeled.Takentogether,ourresultsdemonstratethatECsfromtheaorticfloorseedthetwoaspectsofaorta-associatedhemopoiesisandthattheseECswithhemangioblasticpotentialarepresentonlytransientlyintheaorta.

【 授权许可】

Unknown