Temporary Disruption of the Retinal Basal Lamina and Its Effect on Retinal Histogenesis

Developmental Biology

Manimalha Balasubramani¹  Mark E. Bier¹  Sucai Dong¹  Willi Halfter¹ 

[1] Department of Neurobiology, University of Pittsburgh, 1402 E Biological Science Tower, Pittsburgh, Pennsylvania, 15261

关键词: basal lamina;    chick embryo;    retina;    axonal navigation;   

DOI  :  10.1006/dbio.2001.0396

学科分类：生物科学（综合）

来源: Academic Press

【 摘 要 】

Anexperimentalparadigmwasdevisedtoremovetheretinalbasallaminafordefinedperiodsofdevelopment:thebasallaminawasdissolvedbyinjectingcollagenaseintothevitreousofembryonicchickeyes,anditsregenerationwasinducedbyachasewithmouselaminin-1andα2-macroglobulin.Thelaminin-1wasessentialinreconstitutinganewbasallaminaandcouldnotbereplacedbylaminin-2orcollagenIV,whereasthemacroglobulinservedasacollagenaseinhibitorthatdidnotdirectlycontributetobasallaminaregeneration.Theregenerationoccurredwithin6hafterthelaminin-1chasebyformingamorphologicallycompletebasallaminathatincludedallknownbasallaminaproteinsfromchickembryos,suchaslaminin-1,nidogen-1,collagensIVandXVIII,perlecan,andagrin.Thetemporaryabsenceofthebasallaminahaddramaticeffectsonretinalhistogenesis,suchasanirreversibleretractionoftheendfeetoftheneuroepithelialcellsfromthevitrealsurfaceoftheretina,theformationofadisorganizedganglioncelllayerwithanincreaseinganglioncellsby30%,andtheappearanceofmultipleretinalectopias.Finally,basallaminaregenerationwasassociatedwithaberrantaxonsfailingtocorrectlyentertheopticnerve.Thepresentdatademonstratethatatransientdisruptionofthebasallaminaleadstodramaticandprobablyirreversibleaberrationsinthehistogenesisinthedevelopingcentralnervoussystem.

【 授权许可】

Unknown