Development to the Blastocyst Stage of Pig Embryos Cultured in the Amniotic Fluid of Developing Chick Embryos

Journal of Veterinary Medical Science

Hiroshi SHIMIZU¹  Keita SUZUKI¹  Junji UEDA¹  Marlon B. OCAMPO¹  Lerma C. OCAMPO¹  Tadashi MORI¹  Hiroshi KANAGAWA¹ 

关键词: amniotic fluid;    chick embryo;    swine embryo;   

DOI  :  10.1292/jvms.55.889

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(13)Cited-By(4)Early-stage pig embryos were collected from superovulated donor gilts (60-72 hr post hCG injection) and co-cultured in modified Brinster medium for ovum culture (mBMOC-2) + porcine oviduct epithelial cells (POEC) or in the amniotic fluid of a developing chick embryo (CEAm). The development rate to the compacted morulae or blastocyst stage was significantly higher (P<0.01) in embryos co-cultured in CEAm (60.0%) than those co-cultured in mBMOC-2 + POEC (18.0%). The results indicate that CEAm could be used for production of compacted morulae or blastocysts from early-stage pig embryos. This culture system could also be valuable in situations where a CO2 controlled incubator is not available.

【 授权许可】

Unknown