Altered Susceptibility to Ischemia-Reperfusion Injury in Isolated-Perfused Hearts of Short-Term Diabetic Rats Associated With Changes in Non-enzymatic Antioxidants

The Japanese Journal of Pharmacology

Ho-Yan Yiu¹  Duncan H.F. Mak¹  Michel K.T. Poon¹  Kam-Ming Ko¹ 

[1] Department of Biochemistry, The Hong Kong University of Science & Technology

关键词: Diabetes;    Myocardial ischemia-reperfusion injury;    Glutathione;    Ascorbic acid;    α-Tocopherol;   

DOI  :  10.1254/jjp.85.435

学科分类：药理学

来源: Nihon Yakuri Gakkai Henshuubu / Japanese Pharmacological Society

【 摘 要 】

References(39)Cited-By(4)The effects of short-term (2-week) diabetes on myocardial ischemia-reperfusion (I-R) injury and associated changes in myocardial non-enzymatic antioxidant level were examined. Isolated-perfused hearts prepared from control and diabetic rats were subjected to increasing periods of ischemia and reperfusion, and myocardial I-R injury was assessed by measuring the extent of lactate dehydrogenase (LDH) leakage and contractile force recovery. While a brief period (20 min) of post-ischemic reperfusion caused a smaller extent of LDH leakage, the prolonged period (40 min) of reperfusion produced a greater degree of I-R injury in diabetic hearts, as indicated by the impaired recovery of contractile force. The apparent protection against I-R injury in diabetic hearts during the early phase of post-ischemic reperfusion was associated with increases in myocardial reduced glutathione/ascorbic acid and α-tocopherol levels, with the effect on α-tocopherol being most prominent. Insulin treatment could reverse the diabetes-associated changes in susceptibility to myocardial I-R injury and antioxidant response. The ensemble of results indicates that the myocardium isolated from short-term diabetic rat can produce a beneficial antioxidant response to I-R challenge, which may, in turn, be attributable to the decreased susceptibility to I-R injury observable during the early phase of reperfusion.

【 授权许可】

Unknown