No enzymatic activities are necessary for the stabilization of ascorbic acid by K‐562 cells

FEBS Letters

Esterbauer, Hermann²  Goldenberg, Hans¹  Schweinzer, Esther¹  Waeg, Georg² 

[1] Department of Medical Chemistry, University of Vienna, Waehringerstrasse 10, A-1090 Wien, Austria;department of Biochemistry, University of Graz, Schubertstrasse 1, A-8010 Graz, Austria

关键词: Ascorbic acid;    Dehydroascorbic acid;    Monodehydroascorbate reductase;    K562 cell;    Glutathione;    BSO;    buthionine sulfoximine;    DHA;    dehydroascorbic acid;    GSH;    reduced glutathione;    HPLC;    high-pressure liquid chromatography;    TBSG;    glucose-containing Tris-buffered saline;   

DOI  :  10.1016/0014-5793(93)81691-R

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We disprove that living cells stabilize ascorbate by the activity of a trans plasma membrane semidehydroascorbate reductase. The two processes show different specificities for both substrate and inhibitor. Not only cells but also cell-conditioned buffers stabilize ascorbate as long as compounds with molecular weights above 10 kDa are not removed. The effect is most probably due to chelation of traces of transition metals.

【 授权许可】

Unknown