Gluconeogenesis from ascorbic acid: ascorbate recycling in isolated murine hepatocytes

FEBS Letters

Gyórffy, Erika¹  Braun, László¹  Garzó, Tamás¹  Puskás, Ferenc¹  Csala, Miklós¹  Bánhegyi, Gábor¹  Mandl, József¹ 

[1] Department of Medical Chemistry, Semmelweis University of Medicine, POB 260, H-1444 Budapest, Hungary

关键词: Ascorbic acid;    Dehydroascorbic acid;    Gluconeogenesis;    Glutathione;    Pentose phosphate pathway;    Isolated hepatocyte (Mouse);    BSO;    buthionine sulfoximine;    GSH;    glutathione (reduced form);   

DOI  :  10.1016/0014-5793(96)00654-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Ascorbic acid synthesis and breakdown were investigated in isolated hepatocytes prepared from fasted mice. Stimulation of gluconeogenesis by alanine or xylitol led to ascorbate synthesis. On the other hand, ascorbate or dehydroascorbate addition resulted in concentration-dependent glucose production and elevation of the pentose phosphate pathway intermediate xylulose 5-phosphate. Stimulation of ascorbate oxidation and/or the inhibition of dehydroascorbate reduction increased glucose formation. Inhibition of the pentose phosphate pathway decreased glucose production from dehydroascorbate with increased accumulation of xylulose 5-phosphate. These results suggest that ascorbate can be recycled by a novel way involving intermediates of the pentose phosphate pathway, gluconeogenesis and hexuronic acid pathway.

【 授权许可】

Unknown