Stimulation of In Vitro Angiogenesis by Tetrahydrobiopterin in Bovine Aortic Endothelial Cells

The Japanese Journal of Pharmacology

Tsutomu Nagai²  Toshinori Yamamoto²  Yuji Kiuchi¹  Masako Yasuda²  Masakazu Ishii²  Shunichi Shimizu¹ 

[1] Departments of Pathophysiology, School of Pharmaceutical Sciences, Showa University;Departments of Clinical Pharmacy, School of Pharmaceutical Sciences, Showa University

关键词: Tetrahydrobiopterin;    Angiogenesis;    Proliferation;   

DOI  :  10.1254/jjp.80.177

学科分类：药理学

来源: Nihon Yakuri Gakkai Henshuubu / Japanese Pharmacological Society

【 摘 要 】

References(15)Cited-By(8)This study examined whether tetrahydrobiopterin (BH4) stimulates angiogenesis by measuring the formation of tube-like structures in vascular endothelial cells. Bovine aortic endothelial cells that were cultured between two layers of collagen type I gel formed tube-like networks. Addition of BH4 or sepiapterin, a precursor of BH4 synthesis, stimulated the formation of tube-like structures. The sepiapterin-stimulated tube formation was completely inhibited by co-treatment with N-acetylserotonin, an inhibitor of sepiapterin reductase. These findings show that BH4 stimulates in vitro angiogenesis in vascular endothelial cells.

【 授权许可】

Unknown