Difference in Inhibitory Effects of Dexamethasone and Cyclosporin A on Sephadex Bead-Induced Airway Hyperresponsiveness and Inflammation in Rats

The Japanese Journal of Pharmacology

Katsuo Ikezawa¹  Kazuaki Naito¹  Shigeki Matsubara¹  Hideo Kikkawa¹  Keiko Fushimi¹ 

[1] Lead Optimization Research Laboratory, Tanabe Seiyaku, Co., Ltd.

关键词: Sephadex bead;    Dexamethasone;    Cyclosporin A;    Eosinophil;    Airway hyperresponsiveness;   

DOI  :  10.1254/jjp.77.89

学科分类：药理学

来源: Nihon Yakuri Gakkai Henshuubu / Japanese Pharmacological Society

【 摘 要 】

References(28)Cited-By(3)We investigated the effects of dexamethasone and cyclosporin A on Sephadex bead (Sephadex G-200, Sephadex)-induced airway hyperresponsiveness (AHR) and inflammation in rats. Sephadex (0.5 mg/animal) was intravenously administered on days 0, 2 and 5. Bronchoalveolar lavage, histological study and measurement of AHR were performed on day 7. Dexamethasone (0.1, 1 and 10 mg/kg, p.o. ×3) and cyclosporin A (0.1, 1 and 10 mg/kg, s.c. ×3) clearly inhibited the increase in eosinophils in bronchoalveolar lavage fluid after Sephadex injection. On histological study, pulmonary eosinophilia, granulomatous arteritis with horseshoe-shaped multinuclear giant cell formation and goblet hyperplasia were observed after Sephadex injection. Both dexamethasone (10 mg/kg×3) and cyclosporin A (10 mg/kg×3) inhibited these findings and an increase in eosinophil peroxidase in the lung. Dexamethasone dose-dependently inhibited AHR induced by Sephadex, and completely suppressed it at a dose of 1 mg/kg (×3). Cyclosporin A, however, did not inhibit AHR even at a dose of 10 mg/kg (×3). These results show that there is a difference between dexamethasone and cyclosporin A in the inhibitory effect on Sephadex-induced AHR, and they suggest that eosinophils are not directly associated with the development of AHR after Sephadex injection.

【 授权许可】

Unknown