Significant Roles of Inducible Cyclooxygenase (COX)-2 in Angiogenesis in Rat Sponge Implants

The Japanese Journal of Pharmacology

Osamu Katsumata³  Yoshiteru Harada¹  Masako Isono¹  Izumi Hayashi¹  Shozo Yamamoto²  Ko Hatanaka¹  Masataka Majima¹  Makoto Katori¹  Shohei Yamashina³  Yasuhiro Ikeda¹ 

[1] Department of Pharmacology, Kitasato University School of Medicine;Department of Biochemistry, Tokushima University School of Medicine;Department of Anatomy, Kitasato University School of Medicine

关键词: Angiogenesis;    Cyclooxygenase (COX)-2;    Sponge implant;    Growth factor;    Immunohistochemistry;   

DOI  :  10.1254/jjp.75.105

学科分类：药理学

来源: Nihon Yakuri Gakkai Henshuubu / Japanese Pharmacological Society

【 摘 要 】

References(45)Cited-By(50)Angiogenesis in rat sponge implants, as determined from the concentration of hemoglobin in the sponge granuloma tissues, was gradually increased over a 14-day experimental period. The inducible cyclooxygenase COX-2 was detected in the sponge granuloma tissues at day 4 by Western blot analysis using specific mouse COX-2 antibody. Angiogenesis in the sponge implants was enhanced by daily topical injections of human recombinant basic fibroblast growth factor (bFGF) or human recombinant epidermal growth factor (EGF) (100 or 1000 ng/sponge/day) for 4 days. These treatments clearly enhanced the expression of COX-2 in the sponge granuloma tissues. In immunohistochemical studies, COX-2-positive staining was mainly observed in the endothelial cells of the neovasculature and in the fibroblasts of the granuloma capsule. Administration of the selective COX-2 inhibitor NS-398 (p.o., 3 mg/kg, 3 times a day) for 14 days significantly inhibited the angiogenesis. The angiogenesis enhanced with bFGF or EGF (day 4) was inhibited by administration of indomethacin or NS-398, both in the above regimen, and fell to the level obtained without growth factor treatment. These results suggest that COX-2 induced in the sponge granuloma tissues may participate in neovascularization through prostaglandin formation.

【 授权许可】

Unknown