Possible Involvement of Nitric Oxide in Acetylcholine-Induced Increase of Intracellular Ca2+ Concentration and Catecholamine Release in Bovine Adrenal Chromaffin Cells

The Japanese Journal of Pharmacology

Tetsuo Suemitsu¹  Toshihiro Dohi¹  Takanori Miyasako¹  Shigeo Kitayama¹  Katsuya Morita¹  Yoshiko Uchiyama¹  Naoki Minami² 

[1]Department of Pharmacology, Hiroshima University School of Dentistry

[2]Department of Pharmacology, Nara Medical University

关键词: Nitric oxide;    Catecholamine;    Chromaffin cell;   

DOI  :  10.1254/jjp.65.73

学科分类：药理学

来源: Nihon Yakuri Gakkai Henshuubu / Japanese Pharmacological Society

【 摘 要 】

References(15)Cited-By(27)The role of nitric oxide (NO) in neurotransmitter release was studied using bovine adrenal medullary chromaffin cells. L-Arginine and sodium nitroprusside (SNP) slightly increased the intracellular free calcium concentration ([Ca2+]i), and the effects of the agents were dependent on the presence of the extracellular Ca2+ ([Ca2+]o), but were not blocked by verapamil (30 μM) or diltiazem (30 μM). SNP enhanced the acetylcholine (ACh)-induced rise in [Ca2+]i in the presence but not in the absence of [Ca2+]o. The effects of L-arginine but not those of SNP were inhibited by Nω-nitro-L-arginine (L-NNA). L-NNA significantly reduced the ACh-induced rise in [Ca2+]i and catecholamine (CA) release, and the reduction was restored by L-arginine but not by D-arginine. These results suggest a possible involvement of NO in ACh-induced [Ca2+]i rise and CA release in bovine adrenal chromaffin cells.

【 授权许可】

Unknown