A major role for protein kinase C in calcium‐activated exocytosis in permeabilised adrenal chromaffin cells

FEBS Letters

Burgoyne, Robert D.¹  O'Sullivan, Antony J.¹  Morgan, Alan¹ 

[1] MRC Secretory Control Group, The Physiological Laboratory, University of Liverpool, PO Box 147, Liverpool L69 3BX, England

关键词: Exocytosis;    Secretion;    Chromaffin cell;    Protein kinase C;    Ca2+;    Catecholamine;   

DOI  :  10.1016/0014-5793(88)80246-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The role of endogenously activated protein kinase C in calcium-activated exocytosis was examined in digitonin-permeabilised bovine adrenal chromaffin cells. Protein kinase C activity was reduced by down-regulation following long-term treatment with PMA or by using the inhibitor sphingosine. Both treatments resulted in a substantial reduction in catecholamine secretion elicited by micromolar calcium, indicating that endogenous activation of protein kinase C is a major requirement for calcium-activated exocytosis in chromaffin cells.

【 授权许可】

Unknown