Active Cutaneous Anaphylaxis (ACA) in the Mouse Ear

The Japanese Journal of Pharmacology

Hiroichi Nagai¹  Akihide Koda¹  Toru Miura¹  Naoki Inagaki¹ 

[1] Department of Pharmacology, Gifu Pharmaceutical University

关键词: Active cutaneous anaphylaxis;    Ear (mouse);    Antiallergic drug;    IgE antibody;    IgG1 antibody;   

DOI  :  10.1254/jjp.59.201

学科分类：药理学

来源: Nihon Yakuri Gakkai Henshuubu / Japanese Pharmacological Society

【 摘 要 】

References(37)Cited-By(14)Active cutaneous anaphylaxis (ACA) was studied in the ear of female BALB/c mice. Mice were immunized with ovalbumin in the presence of aluminium hydroxide gel or complete Freund''s adjuvant (CFA). Two weeks after the immunization, ACA was elicited in the mouse ear by injecting 10 μl of antigen solution intradermally into the ear lobe. ACA was assessed by the amount of extravasated dye, which was given intravenously just after the antigen injection. Antiallergic drugs (tranilast, ketotifen and azelastine), antihistamines (chlorpheniramine, diphenhydramine and mequitazine), β-stimulants (isoproterenol and salbutamol), theophylline and glucocorticoids (hydrocortisone, prednisolone and dexamethasone) inhibited the reaction significantly. These drugs inhibited both ACA in mice immunized with alum-precipitated antigen and ACA in mice injected with CFA-emulsified antigen similarly. ACA in the mouse ear might be a useful tool for studying drugs for allergy.

【 授权许可】

Unknown