cDNA cloning, expression and primary structure of Par j I, a major allergen of Parietaria judaica pollen

FEBS Letters

Kennedy, H.¹  Cocchiara, R.¹  Costa, M.A.¹  Falagiani, P.²  Colombo, P.¹  Mistrello, G.²  Venturella, S.¹  Geraci, D.¹  Izzo, V.¹ 

[1] Istituto di Biologia dello Sviluppo, CNR, Via Archirafi 20, 90123 Palermo, Italy;Laboratorio Farmaceutico Lofarma, Milano, Italy

关键词: cDNA;    IgE antibody;    Primary structure;    Pollinosis;    Recombinant allergen;    Parietaria judaica;    Pj;    Parietaria judaica;    CRIE;    crossed radio immunoelectrophoresis;    IPTG;    isopropylthio-β-d-galactoside;   

DOI  :  10.1016/0014-5793(94)80453-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A 659 bp cDNA clone¹ coding for an allergen of Pj pollen has been isolated from a lambda gt11 library, and its DNA sequence determined. The cDNA insert showed an open reading frame of 429 bp coding for an allergenic protein of 14,866 Da and a deduced amino acid sequence containing 143 residues. The expressed recombinant protein represented the major allergen Par j I since it reacted with 95% of the sera from Pj-allergic patients (n = 22) and with two Par j I-specific monoclonal antibodies. No similarity with other known DNA and protein sequences has been detected.

【 授权许可】

Unknown