Defining the Roles of β-catenin and Plakoglobin in Cell-cell Adhesion: Isolation of β-catenin/plakoglobin-deficient F9 Cells

Cell Structure and Function

Satoshi Komiya¹  Akira Nagafuchi¹  Huijie Liu¹  Michio Kawasuji²  Yoshitaka Fukunaga¹  Masayuki Shimizu¹ 

[1] Division of Cellular Interactions, Institute of Molecular Embryology and Genetics, Kumamoto University;Department of Surgery I, Graduate School of Medical Sciences, Kumamoto University

关键词: F9;    β-catenin;    plakoglobin;    targeting;    cadherin;    cell adhesion;   

DOI  :  10.1247/csf.30.25

学科分类：分子生物学，细胞生物学和基因

来源: Japan Society for Cell Biology

【 摘 要 】

References(40)Cited-By(25)F9 teratocarcinoma cells in which β-catenin and/or plakoglobin genes are knocked-out were generated and investigated in an effort to define the role of β-catenin and plakoglobin in cell adhesion. Loss of β-catenin expression only did not affect cadherin-mediated cell adhesion activity. Loss of both β-catenin and plakoglobin expression, however, severely affected the strong cell adhesion activity of cadherin. In β-catenin-deficient cells, the amount of plakoglobin associated with E-cadherin dramatically increased. In β-catenin/plakoglobin-deficient cells, the level of E-cadherin and α-catenin markedly decreased. In these cells, E-cadherin formed large aggregates in cytoplasm and membrane localization of α-catenin was barely detected. These data confirmed that β-catenin or plakoglobin is required for α-catenin to form complex with E-cadherin. It was also demonstrated that plakoglobin can compensate for the absence of β-catenin. Moreover it was suggested that β-catenin or plakoglobin is required not only for the cell adhesion activity but also for the stable expression and cell surface localization of E-cadherin.

【 授权许可】

Unknown