Defining the Roles of α-Catenin in Cell Adhesion and Cytoskeleton Organization: Isolation of F9 Cells Completely Lacking Cadherin-catenin Complex

Cell Structure and Function

Kenji Shimamura³  Kazutaka Ozono²  Satoshi Komiya²  Takaaki Ito¹  Akira Nagafuchi² 

[1] Department of Pathology and Experimental Medicine, Graduate School of Medical Sciences, Kumamoto University;Department of Cellular Interactions, Institute of Molecular Embryology and Genetics, Kumamoto University;Department of Brain Morphogenesis, Institute of Molecular Embryology and Genetics, Kumamoto University

关键词: α-catenin;    F9;    E-cadherin;    ZO-1;    targeting;   

DOI  :  10.1247/csf.11009

学科分类：分子生物学，细胞生物学和基因

来源: Japan Society for Cell Biology

【 摘 要 】

References(51)Cited-By(5)To define the roles of α-catenin in cell-cell adhesion, the E-cadherin, α-catenin, β-catenin, and/or plakoglobin genes were inactivated in F9 teratocarcinoma cells. An E-cadherin-α-catenin fusion protein (Eα) restored full cell-adhesion function and organized the actin-based cytoskeleton and ZO-1, an actin filament binding protein, in F9 cells lacking all endogenous cadherin-catenin complex components. There were two types of cadherin-based cell-adhesion junctions in parental F9 cells, those with ZO-1 and those without ZO-1, and only junctions with ZO-1 were associated with thick actin bundles. Additionally, ZO-1 localized to most Eα-based cell-adhesion junctions. These data demonstrated that Eα supported cadherin-based cell adhesion and recruited actin bundles and ZO-1 to cell-cell contact sites in the absence of cytoplasmic α-catenin. Moreover, the C-terminal half of α-catenin was involved in the formation of cell-adhesion junctions with ZO-1.

【 授权许可】

Unknown