Steryl Glucoside is a Lipid Mediator in Stress-responsive Signal Transduction

Cell Structure and Function

Hirofumi Kai⁴  Ayako Uchiyama²  Kimiko Murakami-Murofushi²  Hiromu Murofushi³  Tetsuyuki Kobayashi²  Shohko Kunimoto²  Wataru Murofushi²  Yoko Ishida²  Susumu Kobayashi¹ 

[1] Faculty of Pharmaceutical Sciences, Science University of Tokyo;Department of Biology, Faculty of Science, Ochanomizu University;Department of Biophysics and Biochemistry, Graduate School of Tokyo University;Department of Pharmaceutical Sciences, Graduate School of Kumamoto University

关键词: stress response;    steryl glucoside;    lipid mediator;    signal transduction;    HSP70;    HSF1;   

DOI  :  10.1247/csf.27.157

学科分类：分子生物学，细胞生物学和基因

来源: Japan Society for Cell Biology

【 摘 要 】

References(34)Cited-By(40)We previously reported that steryl glucoside (SG) is rapidly induced in cells from molds to humans by exposure to environmental stress (Murakami-Murofushi et al. (1997) J. Biol. Chem., 272, 486-489, Kunimoto et al. (2000) Cell Stress & Chaperones, 5, 3-7), and in mold cells SG production is followed by activation of a certain protein kinase and induction of heat shock proteins (HSP) (Maruya et al. (1997) Cell Struct. Funct., 21, 533-538). To determine the biological significance of SG in stress responsive signal transduction, we added SG to the culture of human fibroblasts and examined its effect on HSP induction. We demonstrated a rapid activation of heat shock transcription factor 1 (HSF1) to bind to heat shock element (HSE) and induction of heat shock protein 70 (HSP70) in fibroblast cells by exposure to exogenously added human major SG, cholesteryl glucoside (CG). In addition, enzyme activity to form CG from cholesterol and UDP-glucose was detected in the homogenate of fibroblast cells. These results strongly suggest that CG acts as a mediator in the early stage of stress responsive signal transduction.

【 授权许可】

Unknown