Identification of Negative Regulator of Interleukin-3 (NIL-3) in Bone Marrow

Cell Structure and Function

Kazuhiro John Mori²  Asako K. Sato¹  Kenkichi Sugimoto¹  Yasuhiro Adachi¹ 

[1] Department of Cell Science, Graduate School of Science and Technology;Department of Molecular and Cellular Biology, Faculty of Science, Niigata University

关键词: Interleukin-3;    inhibitor;    apolipoprotein H;    hematopoiesis;   

DOI  :  10.1247/csf.27.81

学科分类：分子生物学，细胞生物学和基因

来源: Japan Society for Cell Biology

【 摘 要 】

References(37)Cited-By(2)We have reported that an inhibitor of interleukin-3 (NIL-3) is produced from murine bone marrow cells in response to excess stimulation of interleukin-3. In this report, we attempted the purification of the NIL-3 activity from bone marrow culture supernatant in the presence of interleukin-3. The purified NIL-3 activity was a protein with relative molecular weight of 54.5 kDa (SDS-PAGE), which inhibited the growth of IL-3 dependent DA-1 cell growth in a dose dependent manner. The N-terminal amino acid sequence of purified NIL-3 activity was determined to be homologous to beta-2 glycoprotein I (apolipoprotein H: APO-H). The gene expression of APO-H was detected by nested-PCR in STIL-3 C5-CM stimulated total bone marrow cells and STIL-3 C5-CM stimulated bone marrow fraction 2 (Fr. 2) which has been reported as a hematopoietic stem cell rich fraction. These observations indicate the possibility that the APO-H is the NIL-3 which was produced from bone marrow cells in response to excess IL-3 stimuli.

【 授权许可】

Unknown