Phospholipid scramblase 1 is required for β2‐glycoprotein I binding in hypoxia and reoxygenation‐induced endothelial inflammation

Journal of Leukocyte Biology: An Official Publication of the Reticuloendothelial Society

关键词: Ischemia and reperfusion;    apolipoprotein H;    eicosanoids;   

DOI  :  10.1189/jlb.3A1014-480R

学科分类：生理学

来源: Federation of American Societies for Experimental Biology

【 摘 要 】

Multiplepathologicconditions,includinghemorrhage,tumorangiogenesis,andischemia‐reperfusionevents,willresultinhypoxiaandsubsequentreperfusion.Previousstudieshaveanalyzedthelipidchangeswithinwholetissuesandindicatedthatischemia‐reperfusionalteredtissueandcellularphospholipids.Usinganinvitrocellculturemodelofhypoxiaandreoxygenation,weexaminedtheendotheliallipidchanges.Wehypothesizedthatphospholipidscramblase1,aproteinthatregulatesbilayerasymmetry,isinvolvedinalteringthephospholipidsofendothelialcellsduringhypoxia,acomponentofischemia,leadingtoβ2‐glycoproteinIandIgMbindingandsubsequentlipid‐mediated,inflammatoryresponses.Wehavecompletedthefirstcomprehensivestudyofsteady‐statephospholipidscramblase1mRNAlevels,proteinexpression,andactivityunderconditionsofhypoxiaandreoxygenation.Phospholipidscramblase1regulatesphosphatidylserineexposureinresponsetooxygenstress,leadingtoβ2‐glycoproteinIandIgMbindingandlipid‐mediated,inflammatoryresponses...

【 授权许可】

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