Formation of Actin Filament Networks in Cultured Rat Hepatocytes Treated with DMSO and Glucagon

Cell Structure and Function

Shuji Takahashi¹  Chihiro Mochizuki²  Hirotoshi Tobioka¹  Masato Saitoh¹  Takashi Kojima²  Yohichi Mochizuki²  Toshihiro Mitaka² 

[1] Department of Pathology, Sapporo Medical University School of Medicine;Department of Pathology, Cancer Research Institute, Sapporo Medical University School of Medicine

关键词: Actin filament networks;    primary rat hepatocytes;    DMSO;    glucagon;   

DOI  :  10.1247/csf.22.269

学科分类：分子生物学，细胞生物学和基因

来源: Japan Society for Cell Biology

【 摘 要 】

References(32)Cited-By(8)Actin filament organization may play an important role in the maintenance of differentiated functions in epithelial cells. We previously reported our success in inducing and maintaining gap junctions, which are two kinds of differentiated function, in primary rat hepatocytes cultured with 2% DMSO and 10-7 M glucagon. In the present study, we demonstrated the formation of actin filament networks in the hepatocytes cultured with 2% DMSO and 10-7 M glucagon. Actin filaments in hepatocytes cultured in medium with only 2% DMSO added from 96 h after plating were concentrated under the plasma membrane and were observed to be circumferential. In hepatocytes cultured in the medium with both 2% DMSO and 10-7 M glucagon added from 96 h, not only the circumferential actin filaments but also the formation of actin filament networks were observed and the networks developed well with time in culture. The networks were observed as a dome-like structure under the cell face and terminated at the circumferential actin filaments. They were composed of electrondense star-like vertices connected by microfilament bundles of varying length and were also very sensitive to the actin disrupter cytochalasin B. However, during the network formation, there were no significant increases in the amounts of actin protein and mRNA. The actin filament networks of the hepatocytes in this culture system might be closely related to the maintenance of differentiated functions.

【 授权许可】

Unknown