Participation of GTP-binding Protein in the Photo-transduction of LParamecium bursaria

Cell Structure and Function

Takao Shinozawa¹  Jun Fujita¹  Yasuo Nakaoka²  Hiroyuki Hashimoto¹ 

[1] Department of Biological and Chemical Engineering, Faculty of Engineering, Gunma University;Department of Biophysical Engineering, Faculty of Engineering Science, Osaka University

关键词: GTP-binding protein;    Photo-transduction;    Rhodopsin-like protein;    Paramecium bursaria;   

DOI  :  10.1247/csf.21.469

学科分类：分子生物学，细胞生物学和基因

来源: Japan Society for Cell Biology

【 摘 要 】

References(15)Cited-By(5)Analysis of the flow of light information in Paramecium, especially the participation of GTP-binding protein, was carried out. Injection of GDP-β-S into Paramecium cell abolished the light-induced inward current. The partially purified rhodopsin-like protein (RLP) that we obtained activated frog rod outer segments (ROS) GTPase. A GTP-binding protein, with Mw 57, 000, was detected by immunostaining with anti-rat Gα rabbit IgG. These results suggest that the light information flows from the light-captured RLP to the GTP-binding protein.

【 授权许可】

Unknown