The photoreceptor protein of Euglena gracilis

FEBS Letters

Passarelli, Vincenzo¹  Barsanti, Laura¹  Gualtieri, Paolo¹  Walne, Patricia L.² 

[1] Istituto di Biofisica, Area della Ricerca San Cataldo, via Alfieri 1, Ghezzano, 56010 Pisa, Italy;Department of Botany, University of Tennessee, Knoxville, TN 37996-1100, USA

关键词: Rhodopsin-like protein;    Photoreceptor;    Optical bistability;    Photocycle;    Euglena gracilis;   

DOI  :  10.1016/S0014-5793(00)02062-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We isolated the photoactive protein Erh, isolated from the photoreceptor of the unicellular photosynthetic flagellate Euglena gracilis. It is a 27 kDa protein with a photocycle resembling that of sensory rhodopsin, but with at least one stable intermediate. We recorded the absorption spectrum of the parent form of this protein both under native form and in the presence of hydroxylamine and sodium borohydride, and the fluorescence spectra of both the parent and intermediate forms. We suggest that Erh is a rhodopsin-like protein and propose a simple photocycle. This protein shows optical bistability, without thermal deactivation.

【 授权许可】

Unknown