Molecular cloning of a diacylglycerol kinase isozyme predominantly expressed in rat retina

FEBS Letters

Kohyama-Koganeya, Ayako²  Hotta, Yoshiki²  Watanabe, Masahiko¹ 

[1] Department of Anatomy, Hokkaido University School of Medicine, Sapporo 060, Japan;Department of Physics, Graduate School of Science, University of Tokyo, Hongo, Bunkyo-ku, Tokyo 113, Japan

关键词: Diacylglycerol kinase (DGK);    PI-signaling;    Photo-transduction;    Retina;    (Rat);    DAG;    diacylglycerol;    PA;    phosphatidic acid;    DGK;    diacylglycerol kinase;    PI;    phosphatidyl inositol;    IP3;    inositol 1;    4;    5-trisphosphate;    PLC;    phospholipase C;    PKC;    protein kinase C;    RT/PCR;    reverse transcription/polymerase chain reaction;   

DOI  :  10.1016/S0014-5793(97)00526-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We have cloned and characterized a new diacylglycerol kinase (DGK) isozyme which is expressed in the retina and the brain of rat. The cDNA contains an open reading frame of 567 amino acid residues with a predicted protein of 64 kDa and shows very high homology to human DGKϵ. The new DGK isozyme contains two distinctive zinc-finger structures and a putative catalytic domain. This DGK expressed predominantly in the inner and outer nuclear layers of retina. This expression pattern is different from those of the previously cloned DGKs including the human DGKϵ, suggesting that this DGK isozyme has potential importance in visual functions as was the case in Drosophila retinal cells.

【 授权许可】

Unknown