Clarification of PC12 Cell Growth Inhibition by a Sialoglycopeptide from Bovine Brain under Low Serum Condition

Cell Structure and Function

Yoko Kobayashi¹  Takao Shinozawa¹ 

[1]Department of Biological and Chemical Engineering, Faculty of Engineering, Gunma University

关键词: sialoglycopeptide;    PC12 cell;    cell-growth inhibition;   

DOI  :  10.1247/csf.19.371

学科分类：分子生物学，细胞生物学和基因

来源: Japan Society for Cell Biology

【 摘 要 】

References(15)Cited-By(1)To study the effect of a bovine brain sialoglycopeptide (SGP) on the nerve cell growth, PC12 cells were used as a model system. Though the inhibition of cell growth, measured by the 3-(4, 5-dimethylthiazol-2-yl) 2, 5-diphenyltetrazolium bromide (MTT) assay, by SGP was slight under the serum concentration of 10%, it was clearly detected under that of 1 to 2%. For the preparation of SGP, filtration through a nitrocellulose membrane was useful.

【 授权许可】

Unknown