Cell Structure and Function | |
Changes in β-Tubulin Isoforms and their RNA Level in Synchronized Tobacco Cells | |
Makoto Nishimura1  Chieko Tanigaki1  Shoji Okamura1  | |
[1] Faculty of Pharmaceutical Sciences, Toyama Medical and Pharmaceutical University | |
关键词: Cell cycle; tubulin isoforms; tubulin mRNA; tobacco; | |
DOI : 10.1247/csf.16.489 | |
学科分类:分子生物学,细胞生物学和基因 | |
来源: Japan Society for Cell Biology | |
【 摘 要 】
References(15)Cited-By(1)Tobacco BY-2 cells were synchronized by an aphidicolin treatment, and their β-tubulin isoforms and their mRNA were analyzed by Western, Northern and dot blottings. The relative ratio of the β-tubulin isoforms changed with the progress of cell cycle stage. By Northern blot hybridization of poly(A)+RNAs with a cloned carrot β-tubulin cDNA probe, a single band of about 1.6 kb was detected throughout the cell cycle. Dot blot hybridization showed that β-tubulin mRNA existed in all stages in the cell cycle at a relatively constant level, though it accumulated slightly more than average at Mphase and decreased during G1 phase.
【 授权许可】
Unknown
【 预 览 】
Files | Size | Format | View |
---|---|---|---|
RO201912080704409ZK.pdf | 623KB | download |