Biosynthesis of Polysaccharide Alginate by Azotobacter vinelandii in a Bubble Column

JOURNAL OF CHEMICAL ENGINEERING OF JAPAN

Takashi Aritomi¹  Kazuhisa Ohtaguchi¹  Kazuhiro Asami¹  Yaik-Sin Tan¹ 

[1] Department of Chemical Engineering, Tokyo Institute of Technology

关键词: Azotobacter vinelandii;    Bubble Column Bioreactor;    Microbial Alginate;   

DOI  :  10.1252/jcej.37.1050

来源: Maruzen Company Ltd

【 摘 要 】

References(22)Cited-By(2)Polysaccharide alginate, which is composed of mannuronic acid (M) and guluronic acid (G), is used as a gel-former and viscosifier in a wide range of applications. The properties of gel-forming, water-binding, and immunogenicity are influenced by the fraction of M- and G-monomers in the alginate. In this study, we engineered an alginate synthesis using culture of Azotobacter vinelandii IAM 9046. The effect of growth conditions on the cell concentration, alginate synthesis, fraction of monomers, etc., were investigated. As a result, alginate with a high content of serial G-monomers was produced in a bubble column reactor. It is our goal to control the monomer fraction of alginate that is required for medical and industrial applications.

【 授权许可】

Unknown