Reconstitution of Azotobacter vinelandii ferredoxin I as a {2[4Fe‐4S]1+/2+} protein

FEBS Letters

Burgess, B.K.²  Stephens, P.J.³  Morgan, T.V.³  Stout, C.D.¹ 

[1] Department of Crystallography, University of Pittsburgh, Pittsburgh, PA 15260, USA;C.F. Kettering Research Laboratory, Yellow Springs, OH 45387 USA;Department of Chemistry, University of Southern California, Los Angeles, CA 90089-0482, USA

关键词: Ferredoxin;    Reconstitution;    Azotobacter vinelandii;    [3Fe] cluster;    O2 lability;    Spectroscopy;   

DOI  :  10.1016/0014-5793(84)80848-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

As normally isolated, Azotobacter vinelandii ferredoxin I (Fd I) is a {[4Fe-4S],[3Fe-3S]} protein: (7Fe)Fd I. We report that anaerobic reconstitution of Fd I from its apoprotein yields a protein whose spectra are distinct from those of (7Fe)Fd I and typical of bacterial ferredoxins. We identify this new form of Fd I as a {2[Fe-4S]} protein: (8Fe)Fd I. (8Fe)Fd I is unstable in air and decomposes to give a ∼ 10% yield of (7Fe)Fd I. These results increase the probability that (8Fe)Fd I is the form of Fd I occurring in vivo and that (7Fe)Fd I results from oxidative degradation during purification.

【 授权许可】

Unknown