Identification of sulfurtransferase enzymes in Azotobacter vinelandii

FEBS Letters

Franchi, E.¹  Kennedy, C.²  Colnaghi, R.¹  Pagani, S.¹ 

[1] Dipartimento di Scienze Molecolari Agroalimentari and Centro Interuniversitaria per lo Studio delle Macromolecole Informazionali, University of Milan, Italy;AFRC Institute of Plant Science Research, Nitrogen Fixation Laboratory, Brighton, BN1 9RQ, UK

关键词: Azotobacter vinelandii;    Sulphurtransferase;    Rhodanese;    3-Mercaptopyruvate sulphurtransferase;   

DOI  :  10.1016/0014-5793(91)80105-C

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Rhodanese and 3-mercaptopyruvate sulphurtransferase have been identified in A. vinelandii. Two distinct active fractions of the two sulphurtransferases were obtained after FPLC ion-exchange chromatography of material partially purified from crude extracts. Rhodanese has been purified to homogeneity, and it consists of one polypeptide chain of M _r ca 25 000. A partial purification of 3-mercaptopyruvate sulphurtransferase was obtained.

【 授权许可】

Unknown